FIG. 3.

Staining for CD3 and hematoxylin and eosin (H&E) staining indicate no persistent inflammatory response to AAV9-CBA-GFP. Histology was assessed 8 weeks after delivery of the vector. (A) and (B) show representative CD3 staining in muscle sections taken from the base of the tongue near the site of AAV9-CBA-GFP injection. No differences were detected between (A) sham-injected and (B) AAV9-CBA-GFP-injected mice. (C) and (D) show H&E-stained tongue myofibers with no indication of an increase in inflammatory cells when comparing (C) sham-injected and (D) AAV9-injected mice. Last, (E) and (F) show CD3 staining in the immediate vicinity of the XII nucleus. No evidence of inflammatory activity was seen in (E) sham-injected and (F) AAV9-injected mice. Scale bars: (A–F) 100 μm. CC, central canal. Color images available online at www.liebertpub.com/hgtb