FIG. 1.

Systems for administration of nebulized perflubron to (A) mice and (B) anesthetized nonhuman primates (pigtail macaques). Perflubron was heated to 82°C and nebulized by blowing oxygen through at 8–10 liters/min. The resulting nebulized material flowed through inert plastic tubing and is visible as a dense fog inside (A) the enclosed Plexiglas box and (B) the nebulization chamber. A metal grate was placed in the chamber to prevent direct contact of the mice with condensed perflubron that pooled on the chamber floor (A). Mice are visible enveloped in the fog, indicated by the arrow in (A). Color images available online at www.liebertpub.com/hgtb