FIG. 6.

Quantitative estimation of the amount of total lung expression of β-Gal activity. Mounted lung sections from each experimental condition were scanned into Adobe Photoshop and processed with ImageJ software. The percentage of total lung area expressing β-Gal activity is graphically depicted. (A) A 1-hr exposure to nebulized perflubron/saline followed by intratracheal AdlacZ instillation immediately, 6 hr, 1 day, or 3 days later. Control mice were naive or were exposed to AdlacZ only or AdlacZ plus liquid perflubron. *p<0.05 versus Naive or AdlacZ only. ^#p<0.05 for 6 hr of perflubron versus all other treated groups. (B) Mice were instilled with AdlacZ 6 hr after exposure to 15, 30, or 60 min of nebulized perflubron. *p<0.05 versus AdlacZ only and versus 15 min and 30 min of nebulized perflubron plus AdlacZ. Data are presented as mean±SEM.