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. 2014 Mar 25;22(5):940–951. doi: 10.1038/mt.2014.31

Figure 5.

Figure 5

ZXL1 enhanced antigen presentation by dendritic cells (DCs). (a,b) Immature mouse DCs (2 × 106 cells/ml) were cultured for 24 hours with LPS (1 µg/ml) or LPS plus iH37Rv (1 × 106 colony forming units (CFUs)/ml)/mannose-capped lipoarabinomannan (ManLAM) (10 µg/ml) in the presence or absence of ZXL1 (5 µmol/l). Supernatants were collected and analyzed for interleukin (IL)-10 and IL-12 production by enzyme-linked immunosorbent assay. (a) IL-10 secretion by Mycobacterium tuberculosis (M. tb) H37Rv/ManLAM-stimulated DCs. *P < 0.05 versus LPS+iH37Rv/ManLAM or LPS+iH37Rv/ManLAM+ZXL4. All data are shown as the means ± SEMs (n = 3). (b) IL-12 secretion by M. tb H37Rv/ManLAM-stimulated DCs. *P < 0.05 versus LPS+iH37Rv/ManLAM or LPS+iH37Rv/ManLAM+ZXL4. All data are shown as the means ± SEMs (n = 3). (c,d) Naive CD4+ T cells were purified from mouse splenocytes using antibody-coupled microbeads and a MACS separator. ZXL1-treated-M. tb H37Rv/ManLAM-pulsed DCs (0.5 × 106) were cocultured with 1 × 106 naive CD4+ T cells for 5 days. IFN-γ and IL-4 production by CD4+ T cells was determined by flow cytometry. (c) IFN-γ production by CD4+ T cells stimulated by DCs pulsed with ZXL1-treated-iH37Rv/ManLAM. (d) IL-4 production by CD4+ T cells stimulated by the DCs pulsed with ZXL1-treated-iH37Rv/ManLAM. IFN, interferon; PE, R-phycoerythrin.