Table 1.

In vivo recombination activity after overexpression of integrases in E. coli BL21(DE3)

Integrase	μg tagged protein/ml 1	% recombination 2
Bxb1	ND	100
Wβ	ND	100
BL3	ND	100
φR4	ND	100
A118	0.6	100
TG1	2.7	100
MR11	2.9	50
φ370	4.1	100
SPBc	9.0	100
TP901-1	32.3	100
φRV	55.9	75
FC1	74.7	100
K38	319.6	100
φBT1	350.4	100
φC31	758.2	100

¹The amount of StrepII tagged integrase detected in a Western blot from 1 ml of culture.

²The % of plasmid extracted from the cultures that had undergone recombination.