Table 2.
Accuracy of integrase mediated site-specific recombination in human HT1080 and mouse ES cells
| Integrase | Sequence | Integrations | Deletions |
|---|---|---|---|
|
BxB1 |
HT1080 |
ES |
|
|
attB |
TCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATCCGGGC |
|
|
|
attP |
GTCGTGGTTTGTCTGGTCAACCACCGCGGTCTCAGTGGTGTACGGTACAAACCCCGAC |
|
|
|
attL |
TCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAACCCCGAC |
3/3 |
|
|
attR |
GTCGTGGTTTGTCTGGTCAACCACCGCGGTCTCCGTCGTCAGGATCATCCGGGC |
7/7 |
2/2 |
| fC31 |
|
|
|
|
attB |
TGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCC |
|
|
|
attP |
GTGCCCCAACTGGGGTAACCTTTGAGTTCTCTCAGTTGGGGG |
|
|
|
attL |
TGCGGGTGCCAGGGCGTGCCCTTGAGTTCTCTCAGTTGGGGG |
3/3 |
|
|
attR |
TTGCCCCAACTGGGGTAACCTTTGGGCTCCCCGGGCGCGTACTCC |
7/7 |
3/3 |
|
R4 |
|
|
|
|
attB |
GCGCCCAAGTTGCCCATGACCATGCCGAAGCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
|
attP |
AGGCATGTTCCCCAAAGCGATACCACTTGAAGCAGTGGTACTGCTTGTGGGTACACTCTGC |
|
|
|
attL |
GCGCCCAAGTTGCCCATGACCATGCCGAAGCAGTGGTACTGCTTGTGGGTACACTCTGC |
3/3 |
|
|
attR |
AGGCATGTTCCCCAAAGCGATACCACTTGAAGCAGTGGTAGAAGGGCACCGGCAGACAC |
2/9 |
1/2 |
| del clone 1 DattR |
AGGCATGTTCCCCAAAGCGATACCAC AAGCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
| del clone 2 DattR |
AGGCATGTTCCCCAAAGCGATACCACTT AAGCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
| del clone 3 DattR |
AGGCATGTTCCCCAAAGCGATACCACTTG GCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
| del clone 4 DattR |
AGGCATGTTCCCCAAAGCGATA GCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
| del clone 5 DattR |
AGGCATGTTCCCCAAAGCGATACCA GTGGTAGAAGGGCACCGGCAGACAC |
|
|
| del clone 6 DattR |
AGGCATGTTCCCCAAAGCGATACCA GAAGCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
| del clone 7 DattR |
AGGCATGTTCCCCAAAGCGATACCAC AAGCAGTGGTAGAAGGGCACCGGCAGACAC |
|
|
| ES del clone 1 DattR |
AGGCATGTTCCCCAAAGCGATACCA AGCAGTGGTAGAAGGGCACCGGCAGACAC |
1/2 |
|
| intn clone 1-3 DattR |
AGGCATGTTCCCCAAAGCGATA CAGTGGTAGAAGGGCACCGGCAGACAC |
3/3 |
|
| fBT1 |
|
|
|
|
attB |
GTCCTTGACCAGGTTTTTGACGAAAGTGATCCAGATGATCCAGCTCCACACCCCGAACGC |
|
|
|
attP |
GGTGCTGGGTTGTTGTCTCTGGACAGTGATCCATGGGAAACTACTCAGCACCACCAATG |
|
|
|
attL |
GTCCTTGACCAGGTTTTTGACGAAAGTGATCCATGGGAAACTACTCAGCACCACCAATG |
3/3 |
|
|
attR |
GGTGCTGGGTTGTTGTCTCTGGACAGTGATCCAGATGATCCAGCTCCACACCCCGAACGC |
7/7 |
2/2 |
| intn clone 1DattR |
GGTGCTGGGTTGTTGTCTC GATCCAGATGATCCAGCTCCACACCCCGAACGC |
1/3 |
|
|
Wβ |
|
|
|
|
attB |
AAGGTAGCGTCAACGATAGGTGTAACTGTCGTGTTTGTAACGGTACTTCCAACAGCTGGCG |
|
|
|
attP |
TAGTTTTAAAGTTGGTTATTAGTTACTGTGATATTTATCACGGTACCCAATAACCAATGAA |
|
|
|
attR |
TAGTTTTAAAGTTGGTTATTAGTTACTGTGATATTTGTAACGGTACTTCCAACAGCTGGCG |
7/7 |
2/2 |
|
SPBc |
|
|
|
|
attB |
AGTGCAGCATGTCATTAATATCAGTACAGATAAAGCTGTATCTCCTGTGAACACAATGGGTG |
7/7 |
2/4 |
|
attP |
AAAGTAGTAAGTATCTTAAAAAACAGATAAAGCTGTATATTAAGATACTTACTAC |
|
|
|
attR |
AAAGTAGTAAGTATCTTAAAAAACAGATAAAGCTGTATCTCCTGTGAACACAATGGGTG |
|
|
| ES del clone 1 DattR |
AAAGTAGTAAGTATCTTAAAAAACA———AGCTGTATCTCCTGTGAACACAATGGGTG |
1/4 |
|
| ES del clone 2 DattR |
aaaatacagcgtttttcatgtacaactatactGCTGTATCTCCTGTGAACACAATGGGTG |
1/4 |
|
|
TP901 |
|
|
|
|
attB |
TGATAATTGCCAACACAATTAACATCTCAATCAAGGTAAATGCTTTTTCGTTTT |
|
|
|
attP |
AATTGCGAGTTTTTATTTCGTTTATTTCAATTAAGGTAACTAAAAAACTCCTTT |
|
|
|
attR |
AATTGCGAGTTTTTATTTCGTTTATTTCAAGGTAAATGCTTTTTCGTTTT |
4/5 |
|
| del clone 1 DattR | AATTGCGAGTTTTTATTTCGTTTATTTC GGTAAATGCTTTTTCGTTTT | 1/5 |
The results show the sequences of the recombinant sites detected following integrase mediate site specific recombination in human HT1080 or mouse ES cells. Underlined and in lowercase in the case of one sequence from a deletion event occurring in ES cells following expression of the SPBc integrase is the sequence of a flanking φ370.1 attP site discussed in the text.