Skip to main content
. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Cell Calcium. 2013 Dec 28;55(2):93–103. doi: 10.1016/j.ceca.2013.12.004

Figure 3.

Figure 3

NAADP-mediated Ca2+ signaling in SKBR3 cells. (A) Representative fluorometric traces of Ca2+ release in single SKBR3 cells induced by the photolysis of caged NAADP. Cells were co-injected with a mixture of the indicated concentration in nM of caged NAADP and the Ca2+ sensitive dye Fluo-4 (200 μM). Free cytosolic Ca2+ was measured after applying 8–10 flashes of UV light to release active NAADP (indicated by dotted line). (B) The % increase from baseline of the peak amplitude of each trace presented in A.