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. 2014 Mar 4;12:12. doi: 10.1186/1478-811X-12-12

Figure 2.

Figure 2

Distribution of NCad in early endothelial cell-cell junctions is regulated by ICAM-2. A- Distribution of ICAM-2, VECad and NCad in HUVEC ~30 hours post siRNA treatment (a-i: control siRNA; d-l: ICAM-2 siRNA). Arrows indicate the gaps between cells that transiently appeared following ICAM-2 inhibition by siRNA. For ICAM-2/VECad co-staining (a-f), see details in Figure 1 legend. Bar = 25 μm B- Quantification of the number of gaps. The gaps were counted manually from 15 fields taken from confocal immunofluorescence images (233 μm x 233 μm, see panel B) in two independent experiments. Results are shown as % of gaps per number of cells per field. Error bars indicate mean ± S.E.M., n = 30. Statistical analysis (t-test: ***p < 0.001). C- VECad and NCad levels are unchanged 30 h post-treatment with ICAM2 siRNA. Quantification of VECad and NCad: Western blot quantification was performed by densitometry, normalized to α-tubulin. Error bars indicate mean ± s.e.m., n = 5.