Skip to main content
. 2014 Jun 1;37(6):1077–1086. doi: 10.5665/sleep.3764

Figure 4.

Figure 4

Increased sleep increases NFκB activity and expression of AMP mRNA. Mean ± SEM luciferase reporter activity (natural log values, arbitrary units) is plotted against time relative to infection with S. marcescens in (A) κB-luc;;MB-switch/UAS-Kir2.1 flies, and (B) κB-luc;;UAS-NaChBac;MB-switch flies treated with RU486 or vehicle. The reading at hour 0 was performed immediately before inoculation. * P < 0.005 and ** P < 0.0001, t-test (Bonferroni corrected). Values from dead flies were discarded from analysis at each time point. N = 112 flies at time ‘0’ for both VEH and RU486; n = 74 VEH, n = 100 RU486 at time ‘24’ in (A); n = 111 at ‘0’ for both VEH and RU486; n = 94 VEH, n = 81 RU486 at time ‘24’ in (B). (C) Mean ± SEM (log(n) transformed) relative mRNA expression levels of drosocin (left panel) and defensin genes (right panel) in RU486 and VEH treated MB-switch>UAS-Kir2.1 flies immediately before infection (BL; baseline) or 18 h after infection (Inf). mRNA expression was normalized to the rp49 gene as an internal control. ** P < 0.005; * P < 0.05, paired t-test, N = 3.