Table 6.
Evaluation of mRNA by real time PCR
| Variables | SHR (n = 10) | SHR-DM (n = 10) | P value |
|---|---|---|---|
| SERCA |
1.00 ± 0.71 |
1.79 ± 1.22 |
0.125 |
| α-MyHC |
1.00 ± 0.60 |
0.72 ± 0.45 |
0.294 |
| β-MyHC |
0.87 (0.70 – 1.17) |
1.11 (0.78 – 1.28) |
0.470 |
| β-MyHC/α-MyHC |
1.20 ± 0.60 |
2.26 ± 0.94 |
0.018 |
| ANP | 1.00 ± 0.38 | 1.88 ± 0.69 | 0.009 |
Data are expressed as mean ± standard deviation or median and 25th and 75th percentile (arbitrary units). SHR: spontaneously hypertensive rats without diabetes mellitus; SHR-DM: SHR with diabetes mellitus. SERCA: sarcoplasmic reticulum calcium ATPase; MyHC: myosin heavy chain; ANP: atrial natriuretic peptide; Cyclophilin: housekeeping gene used in standardizating target gene transcript product quantity. P value: result of statistical test (Student’s t or Mann-Whitney tests).