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. Author manuscript; available in PMC: 2014 May 9.
Published in final edited form as: FEMS Immunol Med Microbiol. 2012 May 25;65(3):467–480. doi: 10.1111/j.1574-695X.2012.00978.x

Fig. 7.

Fig. 7

Probiotic La pretreatment attenuates Smad 7 and NF-kB activation post-Citrobacter rodentium exposure in the colon of mice pups. BALB/c ByJ mice were inoculated bi-weekly after birth with, probiotic La, prebiotic inulin, or a combination of both (synbiotic) and challenged with pathogen Cr at 5 weeks of age. Lysates from the colon were prepared 2 weeks post-Cr infection. Seventy-five micrograms of protein per lane of pooled lysates per treatment (≈ 7.5 μg to 10 μg of protein per mouse) from colonic tissue was analyzed by Western blotting to determine the effects of La, inulin, and synbiotic treatment, and Cr on Smad 7 and IkB-α signaling. Control mice only received a saline vehicle bi-weekly. The data shown here are the representative one of three in vivo experiments performed with N= 7–10 pups per treatment, showing similar results.