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. Author manuscript; available in PMC: 2014 May 14.
Published in final edited form as: Nat Commun. 2013;4:2734. doi: 10.1038/ncomms3734

Figure 6. NEU1 upregulation in 5XFAD reduces β-amyloid and plaques development.

Figure 6

a, b, Injection of an adeno-associated virus containing human NEU1 and PPCA in the hippocampus of 4 and 6 month-old 5XFAD animals, respectively, (5XFAD INJ) reduced the number of amyloid plaques compared to that seen in 5XFAD animals injected only with carrier solution. Plaques were identified by 4G8 immunostaining (brown). a Scale bar 20μm. b Scale bars 10μm. c, Reduction of amyloid plaques in 5XFAD INJ, as determined by quantification of 4G8 immunostaining (mean ± SD; n=4). d and e, NEU1 overexpression in the 5XFAD INJ mice reduces APP as demonstrated by immunoprecipitation (d) and immunoblotting (e) analyses. f, quantification of (e). g, Reduction of β-amyloid levels in hippocampal lysates from 5XFAD INJ mice. h quantification of (g). The 5XFAD/5XFAD-INJ ratio was calculated by densitometric analysis of single bands on immunoblots probed with anti- APP or β-amyloid antibodies and anti-tubulin in 5XFAD and in 5XFAD INJ hippocampal lysates. Asterisks on graphic bars indicate statistically significant results, as determined by the Student’s t test. Data are represented as the mean ± SD (error bars); n>3