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. 2014 May 1;34(5):404–412. doi: 10.1089/jir.2013.0016

FIG. 3.

FIG. 3.

The effect of IFNα2b on endothelial cell proliferation and nitric oxide (NO) bioavailability. (A) IFNα2b had no effect on the number of HAoECs at 24, 48, or 72 h compared to vehicle control. Bars represent the mean and SE of the mean; combined results from n=3 independent experiments. (B) Cellular proliferation as measured by MTT assay shows a positive effect of vascular endothelial growth factor (VEGF) (20 ng/mL) and a negative effect of the NO scavenger PTIO (10 μM) on proliferation in both HAoEC and human venous endothelial cell (HUVEC) after 24 h. The absorbance values were normalized to vehicle-treated cells. (C) IFNα2b (10 ng/mL) had no effect on proliferation compared to vehicle control in HAoEC at 24, 48, or 72 h. (D) In contrast, IFNα2b significantly increased HUVEC proliferation at 24 h (P=0.047). This effect persisted to 48 h but was not statistically significant (P=0.134) at this time point. (E) The NO bioavailability (as measured by supernatant nitrite concentration) was not changed by IFNα2b (0.1–10 ng/mL) at either 6 or 24 h.