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. 2014 May 1;34(5):404–412. doi: 10.1089/jir.2013.0016

FIG. 4.

FIG. 4.

The effect of IFNα2b on endothelial cell tubule network formation. (A) HAoEC formed tubule networks in Matrigel with clear branches (arrowheads) and branch points/junctions (star). Network density was determined by the number of junctions in 3 random fields. Each experimental condition was conducted in triplicate and each graph represents n>3 independent experiments. Bars represent the mean (SE) number of junctions. (B) The density of the HAoEC network was significantly increased by 10 ng/mL VEGF-A (P=0.026) and decreased by 10 μM carboxy-PTIO (P=0.001). IFNα2b (10 ng/mL) had no effect on the HAoEC network density. (C) Similarly, the network density was not changed in HUVEC. Similar positive and negative effects of VEGF-A and PITO were seen in HUVEC (data not shown). (D-i) HAoEC tubule networks in three-dimensional (3D) type 1 collagen matrix were less complex than those on Matrigel. Representative image to show branch points/junctions (star) and branches (arrowheads). (D-ii, iii) Representative images of HAoEC networks in the presence of IFNα2b (10 ng/mL) or vehicle control show no difference in tubule network structure or density.