FIG. 2.

Determination of the most effective miscarriage-inducing dose of mIFN-γ. (A) Overall pregnancy uterine pictures on D8 of pregnancy. (B) Histogram of implantation site numbers in the uterus on D8 with the administration of IFN-γ at different doses. The data at each point were derived from at least 7 separate samples from pregnant mice. **P<0.01. (C) Effects of different doses of IFN-γ on the regulatory T (Treg) cell ratios in the peripheral blood and the spleen. All results are expressed in terms of the % of Treg cells (CD4⁺CD25⁺Foxp3⁺) at different IFN-γ doses. The bars represent the SEM. One-way ANOVA was used to assess the significance of differences. *P<0.05. *Also showed significant differences between the dose of 50,000 IU/mL and other doses (5,000, 25,000, 35,000, and 100,000 IU/mL). There was no significant difference between other compared groups (P>0.05).