Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 1;28(7):735–748. doi: 10.1101/gad.234070.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Herrera-Moyano et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 1. — Genetic interaction with recombination and replication functions of yFACT-deficient cells. (A) Analysis of genetic interactions of spt16-11 (XEI-13) and pob3-7 (EIII-34) mutants with mre11Δ, rad51Δ, and pol32Δ. Single, double, and triple mutants were obtained by genetic crosses, and dissected tetrads were tested for germination on YEPD-rich medium at semipermissive temperature (26°C for pob3-7 and 30°C for spt16-11 crosses). For the spt16-11 and mre11Δ cross, several tetrads were tested for growth at 30°C. (B) Viability of single and double mutants with rad52Δ (R52-9B), rad51Δ (R51-18A), and pol32Δ (P32-1C) in synthetic complete (SC) medium and sensitivity to HU, UV-C, 4-NQO, and MMS by 10-fold serial dilutions are depicted. (C) Recombination frequency using the pLYΔNS system in wild-type (WT; W303-1AR5), spt16-11 (XEI-13), spt16-G132D (XEI-14), pob3-Q308K (XEI-16), and pob3-7 (EIII-34) cells. (D) Recombination frequency in the Lk-AU direct repeat system in wild-type (A3Y3A), spt16-11 (AYS11-1B), spt16-G132D (AYSGD-4A), pob3-Q308K (AYPQK-15B), and pob3-7 (AYP7-7A) cells. (E) Analysis of Rad52 foci formation in wild-type (W303-1A), spt16-11 (DY8107), spt16-G132D (DY5391), pob3-Q308K (DY10308), wild-type (4053-5-2), and pob3-7 (7809-7A) cells. (F) The same as in E in wild-type (R1158), tet-SPT16 (TH-5591), and tet-POB3 (TH-3699) cells in which depletion is induced with 5 μg/mL DOX and in wild-type (YKL83) and spt16-td (YKSD) cells 3 h after degron induction. Mean and standard deviation (SD) of three independent experiments are depicted. (*) P < 0.05 (Student's t-test).