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. 2014 Apr 1;28(7):735–748. doi: 10.1101/gad.234070.113

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© 2014 Herrera-Moyano et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — Analysis of replication in yFACT mutants. (A) Replication intermediates detected by 2D gel electrophoresis in the origin ARS305 and in the 18-kb downstream region C in wild-type (WT; W303-1ARb), spt16-11 (WXEI-48), and pob3-7 (WEIII-36) cells synchronized in G1 with α-factor and released at 30°C. FACS analysis is shown at the bottom. (B) ChIP analysis of BrdU incorporation into the DNA at the 5′ region of LEU2 of the pARSHLB-Leu2 plasmid in wild-type (WRBbL) and spt16-11 (WSRBbL) cells synchronized in G1 and released. Mean and standard error of the mean (SEM) of two independent experiments are shown. (C) Percentage of pARSHLB-Leu2 plasmid loss in wild-type (W303-1ARb), spt16-11 (WXEI-48), and pob3-7 (WEIII-36) cells growing in YEPD-rich medium. Mean and SD of three independent experiments are shown. (*) P < 0.05 (Student's t-test). (D) ChIP analysis of BrdU incorporation into the DNA at genomic regions located 2312 and 417 base pairs (bp) upstream of and 125 and 2502 bp downstream from the ARS1211 replication origin. Other details are as in B.