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. 2014 Apr 1;28(7):735–748. doi: 10.1101/gad.234070.113

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© 2014 Herrera-Moyano et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Effect of R-loop formation in yFACTs mutants. (A) Percentage of wild-type (WT; W303-1ARb), spt16-11 (WXEI-48), and pob3-7 (WEIII-36) S and G2 cells forming Rad52 recombination foci 3 h after a temperature shift from 26°C to 30°C or 37°C with or without RNase H1 (RNH1) overexpression from the pCM189-RNH1 plasmid. (B) Recombination frequency in wild-type (W303-1ARb), spt16-11 (WXEI-48), and pob3-7 (WEIII-36) cells in the plasmid-borne direct repeat system TL-lacZ, which is highly expressed in the absence of DOX. Other details are as in Figure 2A. (C) Recombination frequency using the direct repeats HHF-OUT in wild-type (W303-1ARb), spt16-11 (WXEI-48), and pob3-7 (WEIII-36) cells with or without the hpr1Δ mutation (26°C). (D) Recombination analysis in the Lk-AU direct repeat system in spt16-11 (AYS11-1B), wild-type (A3Y3A), hpr1Δ (AYH-2D), and mft1Δ (AYM-1D) strains plus the double mutants. (E) Percentage of pARSHLB-Leu2 plasmid loss in wild-type (W303-1ARb), spt16-11 (WXEI-48), and pob3-7 (WEIII-36) cells with or without the hpr1Δ mutation growing in YEPD-rich medium. Mean and SD of three independent experiments are shown. (*) P < 0.05 (Student's t-test).