Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Apr 1;28(7):723–734. doi: 10.1101/gad.236869.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Saldaña-Meyer et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 2. — CTCF binds a variety of RNAs in vivo, including Wrap53. (A) PAR-CLIP (top) and immunoblot for CTCF (bottom) in U2OS cells with (+) or without (−) 4-SU incorporation. Whole nuclear lysates were immunoprecipitated with a CTCF antibody or IgG. (B) PAR-CLIP (top) and immunoblots (bottom) for CTCF, DDX5, or SA1 using the same membrane. Immunoprecipitation was performed using an antibody against CTCF in whole nuclear extracts from cells stably transfected with a control shRNA (Ctrl) or shRNA against CTCF (Kd). (C) Genome browser view showing PAR-CLIP tags recovered after deep sequencing over the p53/Wrap53 locus. The CTCF ChIP-seq (chromatin immunoprecipitation [ChIP] combined with deep sequencing) signal from ENCODE is also shown. (D) Graphical representation of normalization of PAR-CLIP tags versus the average relative enrichment obtained by qPCR. (E) Pie chart showing the genome-wide distribution of PAR-CLIP tags.