Fig. 2. Lovastatin inhibits, but exogenous Cho stimulates, Daudi cell cycle progression.

(A) Representative confocal microscopy images of cell cycle analysis with Fucci. Cells in G1 phase show in red whereas cells in S or G2/M phase show in green. (B) Summary plots of G1 phase (red bars) versus S-G2/M phase (green bars) after each treatment. Cells were treated for 72 h with 5 μM lovastatin, 30 μg/ml Cho, 5 μM lovastatin plus 30 μg/ml Cho, TRPC6 shRNA, or 0.5 ng/ml transforming growth factor β (TGF-β, as a positive control because TGF-β is known to induce G1 arrest [20])