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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Biochim Biophys Acta. 2014 Feb 8;1843(5):894–901. doi: 10.1016/j.bbamcr.2014.02.002

Fig. 3. Lovastatin reduces, but Cho elevates, intracellular ROS via gp91-phox NADPH oxidase.

Fig. 3

(A) Representative confocal microscopy images of Daudi cells treated as in Figure 1, with 30 μg/ml Cho plus 250 μM TEMPOL (a mimetic superoxide dismutase), or with 10 mM apocynin. Intracellular ROS levels were indicated with a fluorescent ROS probe, DCF. (B) Summary plots of normalized fluorescence intensity of DCF showing relative ROS levels in Daudi cells after each treatment. (C and D) Representative Western blot of p47-phox and gp91-phox. (E) Normalized p47-phox protein levels in Daudi cells from three separate experiments. (F and G) Normalized cell surface (F) and total (G) gp91-phox protein levels in Daudi cells from three separate experiments.