Table 3.
Identification of majorly expressed P. papatasi salivary proteins by MS
| P. papatasi salivary protein (estimated MW)*/rank | Unused | Sequence coverage† (%) | Accession number | Protein name | Species | No. of unique peptides | Confidence of identification‡ | Local FDR for protein (%) |
|---|---|---|---|---|---|---|---|---|
| 12 kDa | ||||||||
| 1 | 50.41 | 80 | gi 15963505 | 12 kDa salivary protein precursor | P. papatasi | 56 | ∼> 1e-50 | 7e-5 |
| 2 | 25 | 57.8 | gi 15963507 | 14 kDa salivary protein precursor | P. papatasi | 16 | ∼> 1e-25 | 7.3e-5 |
| 3 | 6 | 27.2 | gi 15963515 | 32 kDa salivary protein precursor | P. papatasi | 4 | ∼> 1e-6 | 7.54e-5 |
| 4 | 5.56 | 55.3 | gi 112497698 | 14.2 kDa salivary protein | P. duboscqi | 6 | ∼> 1e-5 | 7.66e-5 |
| 5 | 4 | 72.1 | gi 112497317 | 13.7 kDa salivary protein | P. duboscqi | 22 | ∼> 1e-4 | 7.85e-5 |
| 6 | 2 | 30.5 | gi 112497287 | 14.2 kDa salivary protein | P. duboscqi | 1 | 0.01 | 8.04e-5 |
| 14 kDa | ||||||||
| 1 | 33.3 | 86.1 | gi 335954166 | SP15 protein | P. papatasi | 50 | ∼> 1e-33 | 8.24e-5 |
| 2 | 11.18 | 86.1 | gi 335954098 | SP15 protein | P. papatasi | 44 | ∼> 1e-11 | 8.57e-5 |
| 3 | 6 | 28.2 | gi 15963507 | 14 kDa salivary protein precursor | P. papatasi | 3 | ∼> 1e-6 | 8.66e-5 |
| 4 | 4.05 | 90.9 | gi 112497496 | 14.5 kDa salivary protein | P. duboscqi | 39 | ∼> 1e-36 | 8.76e-5 |
| 5 | 4 | 10.6 | gi 15963515 | 32 kDa salivary protein precursor | P. papatasi | 2 | ∼> 1e-4 | 8.86e-5 |
| 6 | 2 | 39.4 | gi 112361953 | 14.4 kDa salivary protein | P. duboscqi | 3 | 0.01 | 9.05e-5 |
| 7 | 2 | 32.1 | gi 157361529 | 40S ribosomal protein S12-like protein | P. papatasi | 1 | 0.01 | 9.05e-5 |
| 8 | 0.26 | 86.1 | gi 335954106 | SP15 protein | P. papatasi | 40 | 0.55 | 9.55e-5 |
| 26 kDa | ||||||||
| 1 | 30.74 | 71.3 | gi 15963511 | 28 kDa salivary protein precursor | P. papatasi | 36 | ∼> 1e-30 | 8.78e-5 |
| 2 | 28.47 | 70 | gi 15963513 | 30 kDa salivary protein precursor | P. papatasi | 40 | ∼> 1e-28 | 8.85e-5 |
| 28 kDa | ||||||||
| 1 | 30.78 | 57.5 | gi 15963511 | 28 kDa salivary protein precursor | P. papatasi | 26 | ∼> 1e-30 | 4.69e-5 |
| 2 | 23.26 | 60.5 | gi 15963513 | 30 kDa salivary protein precursor | P. papatasi | 18 | ∼> 1e-23 | 4.72e-5 |
| 30 kDa | ||||||||
| 1 | 28.72 | 56.3 | gi 76589378 | 29 kDa salivary protein precursor | P. papatasi | 29 | ∼> 1e-28 | 9.06e-5 |
| 2 | 12 | 37.8 | gi 15963515 | 32 kDa salivary protein precursor | P. papatasi | 8 | ∼> 1e-12 | 9.25e-5 |
| 38 kDa | ||||||||
| 1 | 113.02 | 92 | gi 10443907 | Salivary apyrase | P. papatasi | 114 | ∼> 1e-113 | 4.94e-5 |
| 2 | 6.06 | 74.1 | gi 112496715 | 35.8 kDa salivary protein | P. duboscqi | 27 | ∼> 1e-6 | 5.21e-5 |
| 3 | 2.73 | 32 | gi 299829440 | 32.9 kDa salivary protein | P. sergenti | 7 | ∼> 1e-3 | 5.31e-5 |
| 40 kDa | ||||||||
| 1 | 72.21 | 80.8 | gi 15963517 | 42 kDa salivary protein precursor | P. papatasi | 70 | ∼> 1e-72 | 5.08e-5 |
| 2 | 9.68 | 51.1 | gi 112497236 | Yellow-related protein | P. duboscqi | 29 | ∼> 1e-9 | 5.15e-5 |
| 3 | 7.52 | 69.1 | gi 10443907 | Salivary apyrase | P. papatasi | 6 | ∼> 1e-72 | 5.24e-5 |
| 42 kDa | ||||||||
| 1 | 120.09 | 89.6 | gi 15963517 | 42 kDa salivary protein precursor | P. papatasi | 110 | ∼> 1e-120 | 1.04e-3 |
| 2 | 21.3 | 73 | gi 15963519 | 44 kDa salivary protein precursor | P. papatasi | 13 | ∼> 1e-21 | 1.09e-3 |
| 3 | 15.46 | 65.6 | gi 112497236 | Yellow-related protein | P. duboscqi | 46 | ∼> 1e-15 | 1.11e-3 |
| 4 | 4 | 55.2 | gi 299829410 | Yellow-related salivary protein | P. duboscqi | 19 | ∼> 1e-4 | 1.12e-3 |
| 44 kDa | ||||||||
| 1 | 91.06 | 85.8 | gi 15963517 | 42 kDa salivary protein precursor | P. papatasi | 100 | ∼> 1e-90 | 6.72e-7 |
| 2 | 81.47 | 80.3 | gi 15963519 | 44 kDa salivary protein precursor | P. papatasi | 97 | ∼> 1e-81 | 6.72e-7 |
| 3 | 15.45 | 61.8 | gi 112497236 | Yellow-related protein | P. duboscqi | 49 | ∼> 1e-15 | 6.75e-7 |
| 4 | 6.13 | 59.7 | gi 112497202 | Yellow-related protein | P. duboscqi | 41 | ∼> 1e-6 | 6.76e-7 |
| 46 kDa | ||||||||
| 1 | 130.07 | 98.3 | gi 15963519 | 44 kDa salivary protein precursor | P. papatasi | 188 | ∼> 1e-130 | 1.1e-5 |
| 2 | 43.93 | 77.2 | gi 15963517 | 42 kDa salivary protein precursor | P. papatasi | 41 | ∼> 1e-43 | 1.1e-5 |
| 3 | 8.89 | 74.7 | gi 112497202 | Yellow-related protein | P. duboscqi | 72 | ∼> 1e-8 | 1.11e-5 |
| 4 | 6.02 | 21.9 | gi 76446619 | Yellow-related salivary protein | P. perniciosus | 3 | ∼> 1e-6 | 1.12e-5 |
| 5 | 4.29 | 35.1 | gi 10443907 | Salivary apyrase | P. papatasi | 3 | ∼> 1e-4 | 1.12e-5 |
| 6 | 2.26 | 50.1 | gi 112497236 | Yellow-related protein | P. duboscqi | 26 | ∼> 1e-3 | 1.13e-5 |
| 7 | 2 | 38.7 | gi 52001011 | Yellow-related salivary protein | P. papatasi | 1 | 0.01 | 1.14e-5 |
*
Based on denatured protein bands excised for MS analysis (Figure 1B).
†
Percent representation of a protein based on unique peptide spectral assignments to that protein.
‡
Negative log10 of the unused ProtScore, which is calculated using only peptides from spectra that have not been used by other proteins; a lower value indicates greater confidence of protein identification-based additive assignments of unique peptides.