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. 2014 Mar 1;6:4. doi: 10.1186/2045-824X-6-4

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Copyright © 2014 Chen et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunohistochemical staining for α-SMA in harvested tumor tissues. The results showed that there were fewer α-SMA-positive cells around endothelial cells in the Ang-2-transfected groups (c,d,e) than in non- Ang-2-transfected groups (a,b,f). In addition, this staining showed vessels in c,d,e displayed an aberrant structure characterized by distorted and dilated lumina (vessel indicated by arrow). a: mock, b: control plasmid, c: Ang-2 plasmid, d: Ang-2 + scrambled miRNA, e: Ang-2 + VEGF miRNA, f: VEGF miRNA, g: negative control. Original magnifications: 400× for a,b,c,d,e,f,g.