Figure 1.

Central carbon metabolism and shikimic acid pathways in evolved E. coli PB12.SA22 strain. Glucose transport and phosphorylation are performed by galactose permease (GalP) and glucokinase (Glk), respectively. Genes and coded enzymes: galP, GalP; glk, Glk; tktA, transketolase I; pykF, pyruvate kinase I; pykA, pyruvate kinase II; ppsA, phosphoenolpyruvate synthase; aroF, aroG, aroH, DAHP synthase isoenzymes F, G and H, respectively; aroB, DHQ synthase; aroD, DHQ dehydratase; aroE, shikimate dehydrogenase; aroK, shikimate kinase I; aroL, shikimate kinase II; aroA, EPSP synthase; aroC, chorismate synthase. pJLBaroG^fbrtktA and pTOPOaroBaroD, indicate cloned plasmids for SA overproduction in this strain [10]. Continuous arrows represent unique reactions catalyzed by one or more enzymes; dotted lines or arrows represent two or more enzymatic reactions or incomplete characterized reactions. Cross line in the reaction of SHK to S3P indicates interruption of the SA pathway.