Figure 1. HSCs divide more frequently in female as compared to male mice.
a-c, The frequency of HSCs and MPPs in the bone marrow (a), the total numbers of HSCs and MPPs in two femurs and tibias (b), and the numbers of HSCs and MPPs per gram of body mass (c) did not differ between young adult male and female mice. d, BrdU incorporation into whole bone marrow (WBM) cells, HSCs, and MPPs during a 10 day pulse (a-d, n=5 mice/group in 5 independent experiments). e, H2B-GFP intensity immediately after a 6-week pulse of doxycycline in Col1A1-H2B-GFP; Rosa26-M2-rtTA mice (left) or after a 12-week chase without doxycycline (right). f, The percentages of WBM cells, HSCs, and MPPs that retained H2B-GFP (4 males and 3 females in 3 independent experiments). All data represent mean±standard deviation; *, p<0.05; **, p<0.005; and ***, p<0.0005 by Student’s t-test.