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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nature. 2014 Mar 9;509(7498):49–54. doi: 10.1038/nature13035

Figure 1. Quantifying protein synthesis in haematopoietic cells in vivo.

Figure 1

a, OP-Puro incorporation in bone marrow cells in vivo one hour after administration. b-d, OP-Puro (b), HPG (c), and AHA (d) incorporation in bone marrow cells in culture was inhibited by cycloheximide (CHX). d, Bone marrow cells from mice treated with OP-Puro in vivo exhibited normal AHA incorporation in culture, indicating OP-Puro did not block protein synthesis. e,f OP-Puro versus HPG (e; n=4 mice from 2 experiments) or AHA (f; n=3 mice from 3 experiments) incorporation by haematopoietic cells in culture. g, OP-Puro incorporation in CD150+CD48−LSK HSCs and unfractionated bone marrow cells one hour after administration in vivo. h, Protein synthesis in various haematopoietic stem and progenitor cell populations relative to unfractionated bone marrow cells (n=15 mice from 9 experiments). Extended Data Fig. 1j shows the data from Fig. 1h using a log2 scale. Data represent mean±s.d. Statistical significance was assessed using two-tailed Student’s t-tests (e-f) and differences relative to HSCs (h) were assessed using a repeated measures one way ANOVA followed by Dunnett’s test for multiple comparisons (*, p<0.05; **, p<0.01; ***, p<0.001 relative to bone marrow; ###, p<0.001 relative to HSCs).