Figure 4.

Treatment with PTS2 induces apoptosis in cancer cells. All 4 cancer cell lines were treated with PTS2 (2.5 μg/ml) for 36 h, along with untreated controls. Apoptotic nuclear fragmentation bodies, condensed and dispersed or fragmented particle were stained with blue fluorescence by Hoechst33258 (50 ng/ml) (B) and DAPI (1 μg/ml) (C). Cell morphology (A) was observed in cells treated with PTS2 compared with control cells, using fluorescence microscopy (magnification: × 400).