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. 2013 Nov 5;12:125. doi: 10.1673/031.013.12501

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© 2013

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Immunostaining of hemocytin in the smeared hemolymph from Bombyx mori larvae. Hemocytes were smeared on glass slides soon after puncture of the larvae, fixed, and stained with anti-hemocytin antiserum in combination with Alexa Fluor 488- labeled secondary antiserum and DAPI as described in the Materials and Methods section. (A) A phase contrast microscopy image of smeared hemocytes is shown. (B) A DAPI-stained image of (A) indicating hemocyte nuclei is shown. (C) An Alexa Fluor 488- stained image of (A) indicating hemocytin is shown. The arrowheads point to the fibrous structures connecting the hemocytes. The scale bars indicate 20 µm. g, granulocyte; o, oenocytoid; s, spherulocyte. High quality figures are available online.