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. 2014 Feb 28;12:57. doi: 10.1186/1479-5876-12-57

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Copyright © 2014 Zhang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bufalin inhibits pulmonary metastases. (A) Detection of pulmonary metastases in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups by fluorescence microscopy and necrotic tumor tissues by H&E staining (×400). (B) Detection of intrahepatic, enteric cavity, and abdominal wall tissues by H&E staining (×200). (C) The pixel numbers of pulmonary metastatic foci in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups. (D) The cell numbers of pulmonary metastatic nodules in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups. All data represent the mean ± SD (n = 6). †P < 0.01 vs. control (NS).