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. Author manuscript; available in PMC: 2014 May 9.
Published in final edited form as: Differentiation. 2012 Jul 9;84(2):203–213. doi: 10.1016/j.diff.2012.06.004

Fig. 2.

Fig. 2

V5-tagged Wnt expression. Cell Lysates and media collected from HEK293 (A) and NIH3T3 (B) cells that had been transfected with V5-tagged Wnts (and untagged WNT1 for HEK293 cells) were separated by SDS-PAGE on a 10% gel and then probed with a V5 antibody to determine expression (1st panels) and secretion levels (3rd panels). Cell lysates were also probed for β-Tubulin as a loading control (2nd panels). A V5-tagged protein standard (2A, 5th panel) was processed on a parallel immunoblot at the same time and used to calculate the amount of V5-tagged Wnts secreted into the media of HEK293 cells (2A, 4th panel). In HEK293 cells, WNT9A was the most secreted Wnt into the media (5.7 nM), while WNT2, 8B and 16 were the least secreted Wnts (0.1 nM).