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. Author manuscript; available in PMC: 2014 May 9.
Published in final edited form as: Differentiation. 2012 Jul 9;84(2):203–213. doi: 10.1016/j.diff.2012.06.004

Fig. 4.

Fig. 4

Effect of C-terminal tagging on Wnt activity, expression and accumulation. (A) The indicated untagged and V5-tagged versions of the active Wnts were co-transfected with the STF reporter into HEK293 cells. The C-terminal V5 tag significantly reduced Wnt/β-catenin signaling activity. (B) V5-tagged Wnts 1 and 3A were co-transfected with their untagged counterparts into HEK293 cells to determine whether they can compete for cell surface receptors. V5-tagging affects different Wnts in different manners; it interferes with the ability of WNT1-V5 to bind to cell-surface receptors, but does not do so with WNT3A-V5. (C) The expression levels of Wnts 1 and 3A in lysates (left panel) or their accumulation levels in media (right panel) are not significantly affected by C-terminal V5-tagging in HEK293 cells. Cell lysates were also probed for Lamin as a loading control. Both (A) and (B) are representatives of 3 replicate experiments each.