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. 2013 Oct 20;12:122. doi: 10.1186/1476-4598-12-122

Figure 3.

Figure 3

Loss of mitochondrial potential (ΔΨm) in ZD6474 and UV-B irradiated MDA-MB-468. Cells were stained with Rh-123 after treatment with ZD6474 (Z) and/or UV-B radiation (R) along with untreated control (C). Top panel represented (A) dot plot of treated MDA-MB-468. There was an evident change in ΔΨm in both MCF-7 and MDA-MB-468 in combination treatment as shown by the histogram plot (B). Translocation of bax from cytosol to mitochondria vice versa for cytochrome-c translocation was observed in MDA-MB-468 in combination treatment as confirmed by western blotting (C). COX-IV and α-tubulin was taken as loading control for mitochondrial and cytosolic fraction respectively.