Table 3.
Antimicrobial analyses of F. benjamina against bacterial and fungal strains by disc diffusion assay
|
Microbial strains |
Plant extracts and fractions |
Diameter of inhibition Zone in mm |
||
|---|---|---|---|---|
| Stem | Root | Leaves | ||
|
Bacillus cerus
|
Methanol |
22.5 ± 0.87b |
16.88 ± 0.36ef |
12.00 ± 0.29gh |
|
n-butanol |
22.25 ± 0.72b |
17.73 ± 0.16e |
19.50 ± 0.29cd |
|
| Chloroform |
12 ± 0.58gh |
15.63 ± 0.22f |
10.75 ± 0.14hi |
|
| Ethyl acetate |
17 ± 0.58ef |
16.88 ± 0.07ef |
10.00 ± 0.58i |
|
|
n-hexane |
11.25 ± 0.72hi |
12.99 ± 0.02g |
6.50 ± 0.29j |
|
| Ampiciline |
24.6 ± 0.66a |
18.25 ± 0.43de |
20.75 ± 0.72c |
|
|
Pseudomonas aerugonisa
|
Methanol |
23.5 ± 0.29a |
16.75 ± 0.43de |
12.04 ± 0.31fg |
|
n-butanol |
22 ± 0.87b |
17.23 ± 0.13d |
19.13 ± 0.07c |
|
| Chloroform |
12.25 ± 0.72fg |
15.88 ± 0.36e |
10.5 ± 0.29h |
|
| Ethyl acetate |
16.75 ± 0.72de |
17.13 ± 0.22de |
10.25 ± 0.43h |
|
|
n-hexane |
11 ± 0.58gh |
13.09 ± 0.10f |
7.50 ± 0.29i |
|
| Ampiciline |
24.38 ± 0.79a |
17.75 ± 0.14d |
20.25 ± 0.43c |
|
|
Escherichia coli
|
Methanol |
22.63 ± 0.22b |
16.47 ± 0.59ef |
11.75 ± 0.14h |
|
n-butanol |
22.75 ± 0.43b |
16.23 ± 0.71ef |
18.25 ± 0.43c |
|
| Chloroform |
11.57 ± 0.33h |
15.63 ± 0.22f |
11.58 ± 0.33h |
|
| Ethyl acetate |
16.88 ± 0.65de |
16.88 ± 0.07de |
10.00 ± 0.58i |
|
|
n-hexane |
10.61 ± 0.35hi |
13.34 ± 0.24g |
6.50 ± 0.29j |
|
| Ampiciline |
24.65 ± 0.64a |
17.87 ± 0.71ef |
18.75 ± 0.43c |
|
|
Bacillus subtilis
|
Methanol |
22.5 ± 0.29b |
16.43 ± 0.62e |
12.08 ± 0.29f |
|
n-butanol |
22.83 ± 0.39b |
16.34 ± 0.64e |
18.50 ± 0.29c |
|
| Chloroform |
12 ± 0.58f |
15.75 ± 0.29e |
12.13 ± 0.65f |
|
| Ethyl acetate |
16.63 ± 0.79de |
16.5 ± 0.14de |
10.50 ± 0.29g |
|
|
n-hexane |
10.5 ± 0.29g |
12.96 ± 0.02f |
7.13 ± 0.07h |
|
| Ampiciline |
24.45 ± 0.75a |
17.75 ± 0.14cd |
20 ± 0.51c |
|
|
Aspergillus niger
|
Methanol |
19.25 ± 0.43c |
14.60 ± 0.23e |
12.11 ± 0.29f |
|
n-butanol |
21.13 ± 0.51b |
14.98 ± 0.27e |
19.50 ± 0.29c |
|
| Chloroform |
11.7 ± 0.40f |
17.15 ± 0.66d |
10.38 ± 0.36g |
|
| Ethyl acetate |
16.62 ± 0.22d |
16.38 ± 0.22d |
10.50 ± 0.29g |
|
|
n-hexane |
10.25 ± 0.14g |
14.70 ± 0.40e |
7.63 ± 0.36h |
|
| Terbinafine |
24.38 ± 0.79a |
16.75 ± 0.43d |
20.25 ± 0.43bc |
|
|
Candida albicans
|
Methanol |
18.75 ± 0.72de |
14.88 ± 0.07h |
12.15 ± 0.37i |
|
n-butanol |
21.38 ± 0.36b |
15.23 ± 0.13h |
19.75 ± 0.43cd |
|
| Chloroform |
11.88 ± 0.51ij |
17.44 ± 0.83ef |
10.62 ± 0.22jk |
|
| Ethyl acetate |
16.88 ± 0.07fg |
16.13 ± 0.36gh |
10.38 ± 0.36k |
|
|
n-hexane |
10.63 ± 0.36jk |
14.95 ± 0.55h |
7.88 ± 0.51l |
|
| Terbinafine | 24.5 ± 0.72a | 16.92 ± 0.33fg | 20.25 ± 0.43bc | |
Values are means ± SD of three separate experiments (p < 0.05). Letters in superscript show the significance of the results against a single strain and among various parts.