. 2014 Feb 13;8:12. doi: 10.1186/1752-153X-8-12

Table 4.

Minimum inhibitory concentration (mg/ml) of F. benjamina against bacterial and fungal strains

Microbial strains	Plant extracts and fractions	MIC as mg/ml
		Stem	Root	Leaves
*Bacillus Cerus*	Methanol	1.03 ± 0.03^f	0.96 ± 0.04^f	1.02 ± 0.02^f
	n-butanol	1.79 ± 0.15^bc	1.49 ± 0.02^de	1.54 ± 0.02^cde
	Chloroform	2.16 ± 0.30^a	1.75 ± 0.01^bcd	1.74 ± 0.02^bcd
	Ethyl acetate	1.78 ± 0.15^bc	1.92 ± 0.03^ab	0.99 ± 0.01^f
	n-hexane	1.41 ± 0.15^e	1.54 ± 0.03^cde	1.59 ± 0.01^cde
	Ampiciline	1.03 ± 0.05^f	0.5 ± 0.01^g	0.46 ± 0.03^g
*Pseudomonas aerugonisa*	Methanol	2.11 ± 0.10^cd	2.01 ± 0.01^cde	0.54 ± 0.02^gh
	n-butanol	0.83 ± 0.06^g	2.97 ± 0.05^a	0.77 ± 0.01^gh
	Chloroform	2.49 ± 0.38^b	1.75 ± 0.03^ef	0.86 ± 0.03^g
	Ethyl acetate	2.18 ± 0.19b^c	1.88 ± 0.06^cde	1.95 ± 0.03^cde
	n-hexane	3.10 ± 0.08^a	1.70 ± 0.06^ef	1.55 ± 0.03^f
	Ampiciline	1.85 ± 0.06^de	0.48 ± 0.01^h	0.48 ± 0.01^h
*Escherichia coli*	Methanol	1.94 ± 0.07^c	0.26 ± 0.01ⁱ	0.28 ± 0.02ⁱ
	n-butanol	0.88 ± 0.07^d	0.76 ± 0.01^def	0.73 ± 0.02^d-g
	Chloroform	3.16 ± 0.16^b	0.88 ± 0.01^d	0.47 ± 0.02^ghi
	1Ethyl acetate	`	0.94 ± 0.03^d	0.52 ± 0.02^f-i
	n-hexane	6.51 ± 0.29^a	0.81 ± 0.01^de	0.42 ± 0.02^hi
	Ampiciline	0.34 ± 0.06^hi	0.27 ± 0.01ⁱ	0.26 ± 0.01ⁱ
*Bacillus subtilis*	Methanol	1.84 ± 0.13^b	0.58 ± 0.04^hij	0.55 ±0.02^hij
	n-butanol	0.65 ± 0.06^gh	0.78 ± 0.02^fgh	1.5 ± 0.01^cd
	Chloroform	1.25 ± 0.31^de	3.44 ± 0.05^a	1.68 ± 0.02^bc
	Ethyl acetate	0.95 ± 0.04^ef	1.92 ± 0.07^b	0.95 ± 0.04^efg
	n-hexane	3.42 ± 0.30^a	3.18 ± 0.09^a	1.61 ± 0.02^bc
	Ampiciline	0.41 ± 0.09^ij	0.99 ± 0.01^ef	0.28 ± 0.0^1j
*Aspergillus niger*	Methanol	0.52 ± 0.006^f	0.27 ± 0.006ⁱ	0.52 ± 0.02^fg
	n-butanol	0.74 ± 0.006^e	0.77 ± 0.009^de	0.81 ± 0.03^cd
	Chloroform	0.9 ± 0.006^c	0.84 ± 0.023^c	0.46 ± 0.02^h
	Ethyl acetate	0.98 ± 0.006^a	0.54 ± 0.021^f	0.94 ± 0.03^a
	n-hexane	0.83 ± 0.006^c	0.84 ± 0.015^c	0.77 ±0.01^de
	Terbinafine	0.49 ± 0.006^g	0.28 ± 0.015ⁱ	0.27 ± 0.02ⁱ
*Candida albicans*	Methanol	0.52 ± 0.009^f	0.27 ± 0.009ⁱ	0.51 ± 0.023^f
	n-butanol	0.73 ± 0.006^e	0.77 ± 0.012^d	0.81 ± 0.035^c
	Chloroform	0.89 ± 0.019^b	0.84 ± 0.019^c	0.45 ±0.009^h
	Ethyl acetate	0.97 ± 0.006^a	0.54 ± 0.024^f	0.94 ± 0.020^a
	n-hexane	0.83 ± 0.009^c	0.84 ± 0.019^c	0.77 ± 0.015^d
	Terbinafine	0.49 ± 0.007^g	0.27 ± 0.018ⁱ	0.27 ± 0.012ⁱ

Values are means ± SD of three separate experiments (p < 0.05). Letters in superscript show the significance of the results against a single strain and among various parts. Ampiciline and Terbinafine were used as standards for bacteria and fungi, respectively.