Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1989 Dec 20;8(13):4073–4080. doi: 10.1002/j.1460-2075.1989.tb08591.x

A 6000 kb segment of chromosome 1 is conserved in human and mouse.

S F Kingsmore 1, M L Watson 1, T A Howard 1, M F Seldin 1
PMCID: PMC401584  PMID: 2591369

Abstract

A murine linkage map generated from analyses of 428 meiotic events in an interspecific cross and pulsed field gel electrophoresis allowed examination of the genomic organization of a 6000 kb segment of mouse and human chromosome 1. Analysis of five genes within this syntenic segment of both species revealed striking conservation of gene order, intergenic distance and, to a lesser extent, CpG dinucleotides. In the mouse, meiotic crossover events were not evenly distributed; a hot spot for meiotic recombination was coincident with a CpG-island. These studies provide a practical approach to aid physical mapping of the human genome and a model for determining the molecular principles that govern meiotic recombination. In addition, these findings demonstrate profound conservation of genomic organization over mammalian evolution.

Full text

PDF
4073

Images in this article

4073Image
on p.4073
4074Image
on p.4074
4075Image
on p.4075
4077Image
on p.4077
4077Image
on p.4077
4077Image
on p.4077

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Angel T., Austin B., Catcheside D. G. Regulation of recombination at the his-3 locus in Neurospora crassa. Aust J Biol Sci. 1970 Dec;23(6):1229–1240. doi: 10.1071/bi9701229. [DOI] [PubMed] [Google Scholar]
  2. Bird A. P. CpG-rich islands and the function of DNA methylation. Nature. 1986 May 15;321(6067):209–213. doi: 10.1038/321209a0. [DOI] [PubMed] [Google Scholar]
  3. Brockdorff N., Amar L. C., Brown S. D. Pulse-field linkage of the P3, G6pd and Cf-8 genes on the mouse X chromosome: demonstration of synteny at the physical level. Nucleic Acids Res. 1989 Feb 25;17(4):1315–1326. doi: 10.1093/nar/17.4.1315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown W. R. A physical map of the human pseudoautosomal region. EMBO J. 1988 Aug;7(8):2377–2385. doi: 10.1002/j.1460-2075.1988.tb03082.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Curtis P. J., Palumbo A., Ming J., Fraser P., Cioe L., Meo P., Shane S., Rovera G. Sequence comparison of human and murine erythrocyte alpha-spectrin cDNA. Gene. 1985;36(3):357–362. doi: 10.1016/0378-1119(85)90191-x. [DOI] [PubMed] [Google Scholar]
  6. Dobzhansky T. Translocations Involving the Third and the Fourth Chromosomes of DROSOPHILA MELANOGASTER. Genetics. 1930 Jul;15(4):347–399. doi: 10.1093/genetics/15.4.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Floyd-Smith G., Whitehead A. S., Colten H. R., Francke U. The human C-reactive protein gene (CRP) and serum amyloid P component gene (APCS) are located on the proximal long arm of chromosome 1. Immunogenetics. 1986;24(3):171–176. doi: 10.1007/BF00364745. [DOI] [PubMed] [Google Scholar]
  8. Fulton T. R., Bowcock A. M., Smith D. R., Daneshvar L., Green P., Cavalli-Sforza L. L., Donis-Keller H. A 12 megabase restriction map at the cystic fibrosis locus. Nucleic Acids Res. 1989 Jan 11;17(1):271–284. doi: 10.1093/nar/17.1.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goodfellow P. J., Darling S. M., Thomas N. S., Goodfellow P. N. A pseudoautosomal gene in man. Science. 1986 Nov 7;234(4777):740–743. doi: 10.1126/science.2877492. [DOI] [PubMed] [Google Scholar]
  10. Gutz H. Site Specific Induction of Gene Conversion in SCHIZOSACCHAROMYCES POMBE. Genetics. 1971 Nov;69(3):317–337. doi: 10.1093/genetics/69.3.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hardy D. A., Bell J. I., Long E. O., Lindsten T., McDevitt H. O. Mapping of the class II region of the human major histocompatibility complex by pulsed-field gel electrophoresis. Nature. 1986 Oct 2;323(6087):453–455. doi: 10.1038/323453a0. [DOI] [PubMed] [Google Scholar]
  12. Huebner K., Palumbo A. P., Isobe M., Kozak C. A., Monaco S., Rovera G., Croce C. M., Curtis P. J. The alpha-spectrin gene is on chromosome 1 in mouse and man. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3790–3793. doi: 10.1073/pnas.82.11.3790. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kent R. B., Fallows D. A., Geissler E., Glaser T., Emanuel J. R., Lalley P. A., Levenson R., Housman D. E. Genes encoding alpha and beta subunits of Na,K-ATPase are located on three different chromosomes in the mouse. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5369–5373. doi: 10.1073/pnas.84.15.5369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kinet J. P., Metzger H., Hakimi J., Kochan J. A cDNA presumptively coding for the alpha subunit of the receptor with high affinity for immunoglobulin E. Biochemistry. 1987 Jul 28;26(15):4605–4610. doi: 10.1021/bi00389a002. [DOI] [PubMed] [Google Scholar]
  15. King M. C., Wilson A. C. Evolution at two levels in humans and chimpanzees. Science. 1975 Apr 11;188(4184):107–116. doi: 10.1126/science.1090005. [DOI] [PubMed] [Google Scholar]
  16. Kingsmore S. F., Snoddy J., Choubey D., Lengyel P., Seldin M. F. Physical mapping of a family of interferon-activated genes, serum amyloid P-component, and alpha-spectrin on mouse chromosome 1. Immunogenetics. 1989;30(3):169–174. doi: 10.1007/BF02421202. [DOI] [PubMed] [Google Scholar]
  17. Kingsmore S. F., Vik D. P., Kurtz C. B., Leroy P., Tack B. F., Weis J. H., Seldin M. F. Genetic organization of complement receptor-related genes in the mouse. J Exp Med. 1989 Apr 1;169(4):1479–1484. doi: 10.1084/jem.169.4.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lalley P. A., Davisson M. T., Graves J. A., O'Brien S. J., Roderick T. H., Doolittle D. P., Hillyard A. L. Report of the committee on comparative mapping. Cytogenet Cell Genet. 1988;49(1-3):227–235. doi: 10.1159/000132668. [DOI] [PubMed] [Google Scholar]
  19. Lindsay S., Bird A. P. Use of restriction enzymes to detect potential gene sequences in mammalian DNA. 1987 May 28-Jun 3Nature. 327(6120):336–338. doi: 10.1038/327336a0. [DOI] [PubMed] [Google Scholar]
  20. Mantzouranis E. C., Dowton S. B., Whitehead A. S., Edge M. D., Bruns G. A., Colten H. R. Human serum amyloid P component. cDNA isolation, complete sequence of pre-serum amyloid P component, and localization of the gene to chromosome 1. J Biol Chem. 1985 Jun 25;260(12):7752–7756. [PubMed] [Google Scholar]
  21. Müller U., Stephan D., Philippsen P., Steinmetz M. Orientation and molecular map position of the complement genes in the mouse MHC. EMBO J. 1987 Feb;6(2):369–373. doi: 10.1002/j.1460-2075.1987.tb04764.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Nadeau J. H., Taylor B. A. Lengths of chromosomal segments conserved since divergence of man and mouse. Proc Natl Acad Sci U S A. 1984 Feb;81(3):814–818. doi: 10.1073/pnas.81.3.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ohnishi S., Maeda S., Shimada K., Arao T. Isolation and characterization of the complete complementary and genomic DNA sequences of human serum amyloid P component. J Biochem. 1986 Oct;100(4):849–858. doi: 10.1093/oxfordjournals.jbchem.a121797. [DOI] [PubMed] [Google Scholar]
  24. Patterson M., Schwartz C., Bell M., Sauer S., Hofker M., Trask B., van den Engh G., Davies K. E. Physical mapping studies on the human X chromosome in the region Xq27-Xqter. Genomics. 1987 Dec;1(4):297–306. doi: 10.1016/0888-7543(87)90028-0. [DOI] [PubMed] [Google Scholar]
  25. Petit C., Levilliers J., Weissenbach J. Physical mapping of the human pseudo-autosomal region; comparison with genetic linkage map. EMBO J. 1988 Aug;7(8):2369–2376. doi: 10.1002/j.1460-2075.1988.tb03081.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rappold G. A., Lehrach H. A long range restriction map of the pseudoautosomal region by partial digest PFGE analysis from the telomere. Nucleic Acids Res. 1988 Jun 24;16(12):5361–5377. doi: 10.1093/nar/16.12.5361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Searle A. G., Peters J., Lyon M. F., Evans E. P., Edwards J. H., Buckle V. J. Chromosome maps of man and mouse, III. Genomics. 1987 Sep;1(1):3–18. doi: 10.1016/0888-7543(87)90099-1. [DOI] [PubMed] [Google Scholar]
  28. Seldin M. F., Morse H. C., 3rd, Reeves J. P., Scribner C. L., LeBoeuf R. C., Steinberg A. D. Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group. J Exp Med. 1988 Feb 1;167(2):688–693. doi: 10.1084/jem.167.2.688. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Seldin M. F., Morse H. C., LeBoeuf R. C., Steinberg A. D. Establishment of a molecular genetic map of distal mouse chromosome 1: further definition of a conserved linkage group syntenic with human chromosome 1q. Genomics. 1988 Jan;2(1):48–56. doi: 10.1016/0888-7543(88)90108-5. [DOI] [PubMed] [Google Scholar]
  30. Smith C. L., Lawrance S. K., Gillespie G. A., Cantor C. R., Weissman S. M., Collins F. S. Strategies for mapping and cloning macroregions of mammalian genomes. Methods Enzymol. 1987;151:461–489. doi: 10.1016/s0076-6879(87)51038-2. [DOI] [PubMed] [Google Scholar]
  31. Stahl F. W., Stahl M. M., Malone R. E., Crasemann J. M. Directionality and nonreciprocality of Chi-stimulated recombination in phage lambda. Genetics. 1980 Feb;94(2):235–248. doi: 10.1093/genetics/94.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Steinmetz M., Stephan D., Fischer Lindahl K. Gene organization and recombinational hotspots in the murine major histocompatibility complex. Cell. 1986 Mar 28;44(6):895–904. doi: 10.1016/0092-8674(86)90012-7. [DOI] [PubMed] [Google Scholar]
  33. Symington L. S., Petes T. D. Expansions and contractions of the genetic map relative to the physical map of yeast chromosome III. Mol Cell Biol. 1988 Feb;8(2):595–604. doi: 10.1128/mcb.8.2.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Whitehead A. S., Rits M., Michaelson J. Molecular genetics of mouse serum amyloid P component (SAP): cloning and gene mapping. Immunogenetics. 1988;28(5):388–390. doi: 10.1007/BF00364241. [DOI] [PubMed] [Google Scholar]
  35. Wilson A. C., Carlson S. S., White T. J. Biochemical evolution. Annu Rev Biochem. 1977;46:573–639. doi: 10.1146/annurev.bi.46.070177.003041. [DOI] [PubMed] [Google Scholar]
  36. Woo P., Korenberg J. R., Whitehead A. S. Characterization of genomic and complementary DNA sequence of human C-reactive protein, and comparison with the complementary DNA sequence of serum amyloid P component. J Biol Chem. 1985 Oct 25;260(24):13384–13388. [PubMed] [Google Scholar]
  37. Yang-Feng T. L., Schneider J. W., Lindgren V., Shull M. M., Benz E. J., Jr, Lingrel J. B., Francke U. Chromosomal localization of human Na+, K+-ATPase alpha- and beta-subunit genes. Genomics. 1988 Feb;2(2):128–138. doi: 10.1016/0888-7543(88)90094-8. [DOI] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES