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. 2014 Feb 7;12:7. doi: 10.1186/1477-5956-12-7

Table 1.

Proteins identified and analyzed by MALDI-TOF-TOF/MS

Spot no Protein name Accession no. Deduced apple genome ID Protein MW/PI Pep. count Protein score Relative protein content
Photosynthesis proteins
L8
ribulose-1,5-bisphosphate carboxylase
gi|2961315
MDP0000597996
53314.4/6.14
5
253
Inline graphic
L45
ribulose-1,5-bisphosphate carboxylase/oxygenase activase alpha 2
gi|78100212
MDP0000944409
46944.1/4.84
12
494
Inline graphic
L49
ribulose-1,5-bisphosphate carboxylase/oxygenase activase small protein isoform
gi|115334979
MDP0000321244
47952.1/7.57
10
326
Inline graphic
L50
Oxygen-evolving enhancer protein 2, chloroplastic; Short = OEE2;
gi|11134051
MDP0000361338
28674.3/6.6
4
231
Inline graphic
L51
ribulose 1,5-bisphosphate carboxylase
gi|4206530
MDP0000597996
49888.8/5.94
3
223
Inline graphic
L82
ribulose 1,5-bisphosphate carboxylase
gi|10945633
MDP0000597996
52133/6.18
8
154
Inline graphic
L85
ribulose-phosphate 4-epimerase
gi|225457361
MDP0000137234
30146/8.93
5
533
Inline graphic
L95
ribulose 1,5-bisphosphate carboxylase
gi|371928199
MDP0000597996
49673/6.04
20
498
Inline graphic
L15
Chlorophyll a-b binding protein 3C-like protein
gi|357497757
MDP0000784451
24884.4/5.53
4
133
Inline graphic
L19
putative chlorophyll a/b binding protein
gi|397789264
MDP0000875642
16135/7.82
6
262
Inline graphic
L61
Chlorophyll a-b binding protein 2, chloroplastic;
gi|158562858
MDP0000708928
3798.9/8.2
4
212
Inline graphic
L87
chlorophyll a-b binding protein 8, chloroplastic
gi|225436257
MDP0000866655
29511.2/7.85
2
192
Inline graphic
Amino acid metabolism related proteins
L43
Serine-type peptidase
gi|357495999
MDP000065459
45945.4/6.79
9
267
Inline graphic
L54
precursor of carboxylase p-protein 1, glycine decarboxylase complex
gi|224088838
MDP0000588069
115985/6.51
11
407
Inline graphic
L58
putative plastidic glutamine synthetase
gi|26892040
MDP0000139493
47619.1/6.77
9
457
Inline graphic
Transpot related proteins
L56
Transketolase, chloroplastic
gi|75140229
MDP0000142098
73346.7/5.47
6
20-6
Inline graphic
L81
alanine aminotransferase 3
gi|351724777
MDP0000815368
53789.3/5.52
11
470
Inline graphic
L99
PREDICTED: transketolase, chloroplastic-like
gi|356536526
MDP0000142098
80689.8/6.12
11
299
Inline graphic
L101
Transketolase
gi|357445031
MDP0000142098
80087.5/6.00
10
219
Inline graphic
Energy metabolism related proteins
L23
PREDICTED: ferredoxin--NADP reductase, leaf isozyme, chloroplastic-like isoform 1
gi|356538289
MDP0000811918
40803.8/8.7
14
477
Inline graphic
L24
NAD dependent epimerase/dehydratase
gi|255559448
MDP000019372
43230.8/8.75
6
243
Inline graphic
Carbohydrate metabolism related proteins
L27
PREDICTED: triosephosphate isomerase
gi|225449541
MDP0000152242
27396.2/6.34
5
373
Inline graphic
L33
alcohol dehydrogenase
gi|307135978
MDP0000239956
40657.6/8.53
3
138
Inline graphic
L36
cytosolic malate dehydrogenase
gi|78216493
MDP0000174740
35970.5/6.01
3
352
Inline graphic
L40
glyceraldehyde-3-phosphate dehydrogenase A
gi|381393060
MDP0000527995
43224.5/8.1
18
853
Inline graphic
L41
PREDICTED: fructose-1,6-bisphosphatase, cytosolic
gi|225460680
MDP0000251810
37554/6.03
7
332
Inline graphic
L42
glyceraldehyde-3-phosphate dehydrogenase B
gi|381393062
MDP0000835914
48490.9/8
21
790
Inline graphic
L46
glyceraldehyde 3-phosphate dehydrogenase A subunit
gi|62318887
MDP0000527995 c
17730.1/5.35
6
210
Inline graphic
L47
NADP-dependent glyceraldehyde-3-phosphate dehydrogenase;
gi|3913711
MDP0000152497
53654.6/6.76
10
375
Inline graphic
L53
alcohol dehydrogenase
gi|307135978
MDP0000239956
40657.6/8.53
8
216
Inline graphic
L59
chloroplast sedoheptulose-1,7-bisphosphatase
gi|118175929
MDP0000244771
42824.9/6.06
9
377
Inline graphic
L83
PREDICTED: triosephosphate isomerase
gi|225449541
MDP0000152242
27396.2/6.34
6
266
Inline graphic
L86
PREDICTED: pyruvate dehydrogenase E1 component subunit beta
gi|357159289
MDP0000146411
40081.6/5.47
5
407
Inline graphic
Binding proteins
L68
ATP synthase, subunit B
gi|7578491
MDP0000565338
53365/5.57
15
752
Inline graphic
L69
ATP synthase subunit B
gi|91983091
MDP0000565338
51812.1/5.27
21
703
Inline graphic
L71
ATP synthase, subunit B
gi|7578491
MDP0000565338
53365/5.57
19
791
Inline graphic
L72
putative ATP synthase subunit B
gi|56784991
MDP0000565338
45936.9/5.33
17
971
Inline graphic
L73
PREDICTED: ATP synthase subunit B, mitochondrial-like
gi|225424142
MDP0000565338
59699.2/5.84
18
760
Inline graphic
L77
ATP synthase, subunit B
gi|7578491
MDP0000565338
53365/5.57
19
632
Inline graphic
L78
ATP synthase CF1 alpha subunit
gi|346683279
MDP000092905
55443.9/5.09
21
1100
Inline graphic
L79
ATP synthase subunit B
gi|91983091
MDP0000565338
51812.1/5.27
24
923
Inline graphic
Antioxidant related proteins
L25
Probable aldo-keto reductase 2
gi|378548276
MDP0000228499
38436.6/6.17
6
341
Inline graphic
L37
aldo/keto reductase AKR
gi|62526573
MDP0000228499
38026.5/6.38
10
483
Inline graphic
Defense and stress related proteins
L13
class III endo-chitinase
gi|33347391
MDP0000280265
17774.6/4.12
1
207
Inline graphic
L16
heat shock protein 70
gi|30962610
MDP0000322220
47584/5.1
9
106
Inline graphic
L17
beta-1,3-glucanase
gi|399137110
MDP0000570395
37551.2/5.07
4
258
Inline graphic
L18
thioredoxin peroxidase
gi|21912927
MDP000020081
30103.4/8.2
5
328
Inline graphic
L22
thaumatine-like protein
gi|20149274
MDP0000552328
22948.5/4.6
7
317
Inline graphic
L28
ascorbate peroxidase
gi|145581388
MDP0000199034
27711.8/5.53
6
414
Inline graphic
L29
ascorbate peroxidase
gi|319993039
MDP0000194474
12064.3/8.72
3
73
Inline graphic
L30
early-responsive to dehydration 2
gi|383100964
MDP0000322220
69263.1/5.19
11
149
Inline graphic
L84
quinone oxidoreductase [Gymnadenia conopsea]
gi|89276317
MDP0000393227
21631.1/6.06
2
159
Inline graphic
L89
phi class glutathione S-transferase protein
gi|329130898
MDP0000266097
23996.3/5.97
3
90
Inline graphic
L90
IgE-binding protein MnSOD
gi|10862818
MDP0000187714
22957.7/6.06
6
397
Inline graphic
Unknown proteins
L20
uncharacterized protein LOC100805310
gi|359806638
MDP0000875642
27959.1/5.29
9
353
Inline graphic
L31
PREDICTED: uncharacterized protein At2g37660, chloroplastic
gi|225440390
MDP0000176370
27681.5/5.85
2
150
Inline graphic
L32
predicted protein
gi|224087915
MDP0000641719
27403.9/5.74
9
464
Inline graphic
L38
PREDICTED: clavaminate synthase-like protein At3g21360
gi|225442460
MDP0000406399
36588.7/6.1
4
80
Inline graphic
L57 predicted protein gi|224138316 MDP0000148186 45396.1/6.11 14 534 Inline graphic

*In relative protein content graph, from left to right were Inline graphic: Control group 3 days post-treatment, Inline graphic: Inoculated group 3 days post-treatment, Inline graphic: Control group 6 days post-treatment, Inline graphic: Inoculated group, 6 days post-treatment respectively.