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. 2014 Mar 7;13:50. doi: 10.1186/1476-4598-13-50

Figure 4.

Figure 4

SW IV-134 engages in SMAC-dependent pathway activation. (A) SKOV3 cells were treated with vehicle only (Ctrl.), SW43 (10 μM), and SW IV-134 (10 μM) for 4 hours and cell lysates were subjected to Western blot analysis using antibodies against XIAP, cIAP-1, cIAP-2, NF-қB and NIK. While XIAP seems unaffected under the conditions used, cIAP-1 and cIAP-2 proteins are readily undetectable shortly post treatment. SW IV-134 treatment also leads to accumulation of NF-қB inducing kinase (NIK) and activation of NF-қB pathway. (B) SKOV3 cells were treated with SW IV-134 at the indicated doses either alone or in the presence of proteasome inhibitor MG132 (20 μM) for 18 hours. Cell death induction is partially rescued by MG132 and suggests a requirement for an intact proteasomal machinery to achieve efficient tumor cell killing. Cell viability was determined as described in the experimental methods (p < 0.011, Student’s t-test).