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. 2014 May 9;9(5):e96377. doi: 10.1371/journal.pone.0096377

Figure 1. Time course of mRNA expression in TK-173 cells cells treated with 100 nM tacrolimus.

Figure 1

Expression of mRNA for NAD(P)H-oxidase 4 (A), transgelin (B), procollagen α1(V) (C), and alpha-smooth muscle actin (D) in untreated cells (open columns), and cells treated with 100 nM tacrolimus (filled). Cells were switched to serum-free medium on day -1, and were then exposed to 100 nM tacrolimus starting at day 0. Cell samples were collected from day 0 to day 5. All results were normalized to day 0. Cells showed a significant response to tacrolimus after one day (Nox4 and transgelin) or two days (tropomyosin-1), respectively. Alpha-smooth muscle actin mRNA showed a slow up-regulation in both treated and untreated cells, most likely as a reaction to serum deprivation. (*) denotes significant (p≤0,05) difference between tacrolimus-treated and control cells at the same timepoint.