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. 2014 May 9;9(5):e96377. doi: 10.1371/journal.pone.0096377

Figure 2. Induction of fibrosis-related genes in TK-173 fibroblasts by nanomolar levels of tacrolimus.

Figure 2

Exposure to increasing concentrations of tacrolimus (0–1000 nM) induced mRNA expression in a concentration-dependent manner after three days for Nox4 (A), transgelin (B), tropomyosin-1 (C), procollagen α1(V) (D), and transforming growth factor β-1 (E). a-smooth muscle actin mRNA expression (F) was not affected. RT-qPCR results were standardized to 18S rRNA, linearized, and normalized to the control group (without tacrolimus). (*) indicates significance from untreated cells p≤0,05.