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. 2014 May 9;9(5):e96377. doi: 10.1371/journal.pone.0096377

Figure 6. Effect of siRNA-mediated Nox-4 knock-down in human TK-173 fibroblasts.

Figure 6

Cells were transfected with Nox4-targeted siRNA (siNOX4), or non-target siRNA (si(-)) as a control, resp., and exposed to 100 nM tacrolimus, or control conditions, for three days. Transfection with Nox4-targeted siRNA reduced Nox4 mRNA expression by 61% (untreated control), and 64% (100 nM tacrolimus), resp., compared to non-target siRNA transfected cells (A). Nox4 knock-down did not have significant effects on transgelin (B), tropomyosin-1 (C), and alpha-smooth muscle actin (D) mRNA expression, but induced a significant up-regulation of mRNA for procollagen α1(V) in untreated cells, and a down-regulation to control levels in tacrolimus-treated cells (E). Results were normalized to control-transfected, untreated cells. (*) denotes significant (p≤0,05) differences between control-transfected and siRNA-transfected cells.