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. Author manuscript; available in PMC: 2014 May 9.
Published in final edited form as: J Immunol. 2008 Mar 1;180(5):2933–2941. doi: 10.4049/jimmunol.180.5.2933

FIGURE 1.

FIGURE 1

Impaired CD8+ T cell memory in FasL mutant mice. C57BL/6J WT mice, or mice with mutation/deficiency in inducible NO synthase-2, IFN-γ, TNFR-αβ, FasLgld/gld, IL-12, and CD40L were infected with rLM-OVA (1 × 104 i.v.). Spleen cells were restimulated (30 × 106 cells) on days 7 and 30 (B) or day 60 (A and B) with irradiated OVA-expressing EG-7 cells (5 × 105) and IL-2 (0.1 ng/ml). Five days later, cells were harvested and evaluated for cytolytic activity against EL4 targets in the presence or absence of the CTL epitope OVA257–264. Percentage of cytotoxicity in the absence of OVA257–264 ranged from 0 to 10%. Figure is representative of three such experiments conducted.