FIGURE 12.

Impaired cytokine expression in FasL mutant mice. WT and FasL mutant mice were infected with LM-OVA. At day 75, spleens were removed and snap frozen in dry-ice ethanol bath. Total RNA was extracted, and 5 μg of RNA was taken to make cDNA. The amount of RNA for various cytokines and β-actin was measured using the SYBR Green method of quantification, as outlined in Materials and Methods. Values represent the levels of cytokine mRNA relative to β-actin mRNA. Although differences in several cytokines were noted between WT vs FasL mutant spleens, only the difference in the relative expression of IFN-γ achieved significance (*, p < 0.05) by Mann-Whitney U test.