FIGURE 5.

Impaired maintenance of CD8⁺ T cell memory in FasL mutant mice. WT and FasL mutant mice were infected with 10⁴ LM-OVA. Ninety days after infection, CD8⁺ T cells were purified from spleens of infected mice and adoptively transferred (1–5 × 10⁶/mouse) into naive WT and FasL mutant mice. Cell numbers were adjusted to inject exactly the same numbers of OVA-specific CD8⁺ T cells. Sixty days after cell transfer, spleens were removed from recipient mice and tested for cytolytic activity against OVA_257–264, as described in Fig. 1.