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. 2013 Oct 26;13:90. doi: 10.1186/1472-6750-13-90

Figure 7.

Figure 7

Validation of violuric acid HTS assay. Oxidation of 20 mM violuric acid (VIO) by MtL (white bars) and TvL (black bars) using equal laccase activity units respecting ABTS (A). Changes in the visible spectrum of 20 mM VIO after oxidation (t0, dashed lines; 20 h, continuous lines) by different volumes of crude cell extracts from S. cerevisiae micro-cultures secreting 3A4 HRPL (the inset shows the oxidation of VIO by 100 mU TvL in 0, 1 and 20 h) (B). Linearity of the HTS assay using crude extracts from S. cerevisiae micro-cultures secreting 3A4 HRPL (triangles) or R2 LRPL (squares) (C). Reproducibility of the endpoint HTS assay based on the oxidation of 20 mM VIO using crude cell extracts from replicates of the same clone expressing 3A4 HRPL (20 μL supernatant). The activities are plotted against the clones in descending order (D).