Table 2.
Suppression of microtubule dynamic instability in MCF7-EGFP-α-tubulin cells incubated with vehicle, taccalonolide AJ or Paclitaxel
| |
Vehicle |
25nM AJ |
50nM AJ |
100nM AJ |
50nM Paclitaxel |
150nM Paclitaxel |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Value ± SEM | Value ± SEM | % Change | Value ± SEM | % Change | Value ± SEM | % Change | Value ± SEM | % Change | Value ± SEM | % Change | |
| Growth rate (μm/min) |
7.5±0.25 |
7.2±0.3 |
−5.1 |
6.9±0.3 |
−8.50 |
6.5±0.3 |
−13.3** |
7.2±0.3 |
−3.9 |
6.5± 0.3 |
−13.3** |
| Shortening rate (μm/min) |
13.9±0.9 |
12.0±0.8 |
−14.0 |
8.6±0.4 |
−38.5*** |
9.1±0.9 |
−34.9*** |
12.0±0.7 |
−13.9 |
8.0±0.5 |
−42.5*** |
| Percent Time Growing |
35.8 |
29.2 |
−18.6 |
14.9 |
−58.3 |
14.9 |
−58.4 |
26.0 |
−27.5 |
18.2 |
−49.3 |
| Percent Time Shortening |
16.6 |
13.7 |
−17.3 |
9.7 |
−41.6 |
9.7 |
−41.7 |
11.5 |
−31.1 |
7.8 |
−53.4 |
| Percent Time Attenuated |
47.6 |
57.1 |
20.1 |
75.4 |
59.0 |
75.4 |
58.6 |
62.6 |
31.6 |
74.1 |
55.8 |
| Catastrophe Frequency (per min) |
1.8±0.1 |
1.6±0.2 |
−8.9 |
1.2±0.1 |
−32.8*** |
1.2±0.1 |
−36.1*** |
1.4±0.1 |
−21.1* |
1.3±0.2 |
−30.0* |
| Rescue Frequency (per min) |
10.4±0.5 |
10.5±0.6 |
0.6 |
11.6±0.7 |
11.4 |
11.0±0.7 |
5.9 |
11.2±0.6 |
7.5 |
12.8±0.6 |
22.8** |
| Dynamicity |
5.1 |
3.8 |
−25.3 |
1.9 |
−62.3 |
1.9 |
−62.1 |
3.3 |
−34.1 |
1.9 |
−62.33 |
| MTs/Cells Counted |
40/18 |
41/13 |
41/13 |
31/12 |
47/14 |
30/13 |
|||||
| % Dynamic MTs | 65 | 51 | 41 | 32 | 54 | 44 | |||||
*P < 0.05; **P < 0.01; ***P < 0.001 as determined with a Student’s t-test.