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. 2014 May 9;9(5):e96697. doi: 10.1371/journal.pone.0096697

Figure 1. Serum titer evaluation of idiotype antibody-immunized mouse.

Figure 1

The BALB/c mouse was immunized with anti-CD22 SM03 as described in Methods. Serum was collected at the fourth day after the second boost dose, and serum titer was evaluated by ELISA. Antigens (0.1 µg/well) that were coated onto the ELISA plate are chimeric anti-CD22 MAb (▪), murine anti-CD22 MAb (▴), chimeric anti-TNFα MAb (⧫), and BSA (▾). Results are expressed as means ± SEM of duplicates. Data shown is a representative of three separate experiments with similar results.