Figure 3. Binding specificity of selected scFv phage clones.

Binding specificity of selected phage clones derived from panning against chimeric (A) or murine (B) anti-CD22 MAb were examined by phageELISA as described in Methods. Freshly prepared phages were incubated with 0.1 µg/well of chimeric anti-CD22 MAb (column bars with diffuse dots pattern), murine anti-CD22 MAb (column bars with chessboard pattern), humanized anti-CD20 MAb (column bars with horizontal lines pattern), chimeric anti-TNFα MAb (open column bars), or BSA (column bars with diagonal lines pattern) for 1 h, and bound phages were detected with HRP-conjugated anti-M13. Results are expressed as means ± SEM of duplicates. Data shown is a representative of three separate experiments with similar results.