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. 2014 May 9;9(5):e96697. doi: 10.1371/journal.pone.0096697

Figure 5. Affinity determination of anti-idiotype scFv Hc5 by surface plasmon resonance.

Figure 5

The idiotype chimeric anti-CD22 MAb was immobilized onto Biacore CM5 chip and the anti-Id scFv Hc5 was then applied to determine the binding affinity as described in Methods. (A) Kinetic binding of anti-Id scFv Hc5 was measured by surface plasmon resonance using the BIAcore 3000 system. Numbers in the right margins indicate the concentrations of anti-Id scFv used for each curve. Data shown is a representative of two separate experiments with similar results. (B) The association rate (k on), dissociation rate (k off) and affinity constant (K D) were derived using BIAevaluation software from two separate experiments.