Table 1. Enrichment of phages by four consecutive rounds of panning.
Target Antibody | Selection Round | Input Titera | Output Titera | O/I Ratio | Enrichment Factorb |
Chimeric | 1 | 3.0×1013 | 6.7×104 | 2.2×10−9 | 1 |
2 | 8.6×1012 | 3.8×105 | 4.5×10−8 | 20 | |
3 | 1.1×1013 | 6.1×105 | 5.8×10−8 | 26 | |
4 | 6.4×1012 | 2.1×107 | 3.3×10−6 | 1500 | |
Murine | 1 | 3.0×1013 | 1.0×106 | 3.3×10−8 | 1 |
2 | 4.2×1012 | 8.5×104 | 2.0×10−8 | 0.6 | |
3 | 9.8×1012 | 2.9×106 | 2.9×10−7 | 9 | |
4 | 4.5×1012 | 3.1×107 | 7.0×10−6 | 212 |
Titers are shown as colony-forming unit (cfu)/ml.
Enrichment factor is defined as:
[O/I Ratio of selection round N]/[O/I Ratio of the first selection round].