Figure 2.

Detection of STLV-1 tax and STLV-1 bZIP factor (SBZ) transcripts and their splicing junctions. STLV-1 tax and SBZ transcripts were amplified by RT-PCR using the primers flanking the putative splicing site. The bands of the amplified fragments are shown together with the corresponding transcript of HTLV-1 in the images of agarose gel stained with ethidium bromide. Numbers in the scheme indicate the nucleotide positions of HTLV-1 ATK provirus. Sequences of the amplified STLV-1 tax and SBZ transcripts are represented with uppercase letters and aligned with a reference sequence of HTLV-1 (ATK). The lowercase letters represent the intron region of HTLV-1 or STLV-1 provirus.